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In vitro transcription for synthesis of mRNA
- 5mM CaCl2
a dimethylformamidyl (dmf) base-protecting group on guanine
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RNAs containing 5moU have been shown to exhibit reduced innate immune activation and altered translational properties compared with unmodified uridine
DNase I (RNase-free) Amount/Package:1kU / centrifuge tube In vitro transcription for synthesisProduct Information DNase I (RNase free) is an endonuclease that digests single and double stranded DNA. It nonspecifically cleaves DNA to release di ,tri and oligonucleotide products with 5' phosphorylated and 3' hydroxylated end. Source: Recombinant E. coli Concentration: 1U L Unit Definition: One unit is defined as the amount of enzyme required to completely degrade 1g of DNA in 10 minutes at 37. Storage Buffer: 50mM Tris HCl (pH7. 5, 25), 10mM
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