Mouse IFN ELISA Kit Tag & Loading Control Antibody Replace the medium with DMEM

Replace the medium with DMEM supplemented with 2% FBS (to reduce the impact of cytokines in the serum)

MacFarlane M

5 4 5 5 μmol/L standard (μL) 0 40 60 80 100 140 160 200 Reagent One (μL) 200 160 140 120 100 60 40 0 3

and the β chain is shared with IL-3 and IL-5 receptor

CD40L self-assembles into functional trimers which induces CD40 trimerization and downstream signaling

Mouse IFN ELISA Kit Tag & Loading Control Antibody Replace the medium with DMEMProduct Specification Usage Experimental equipment required for the experiment: 1. Microplate reader (450nm) 2. High precision pipette and gun tips: 0. 5 10uL, 5 50uL, 20 200uL, 200 1000uL 3. 37 constant temperature box 4. Distilled water or deionized water Sample processing and requirements: Serum: Place the whole blood sample collected in the serum separation tube at room temperature for 2 hours or at 4 overnight, then centrifuge at 1000g for 20