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5) Read the fluorescent signal on a fluorescence microplate reader [Note 5]
and organ formation
Blank control Sample Assay buffer 40ul 40ul Sample to be measured 0ul 50ul Lysate 50ul 0ul Ac-YVAD-pNA (2 mM) 10ul 10ul Total Volume 100ul 100ul
Species Human Synonyms Beta-2-Microglobulin
and HRP enzyme conjugate were sequentially added to microwells pre-coated with mouse type I collagen cross-linked carboxy terminal peptide (CTX I) antigen
Alexa Fluor® 488 Rat Anti-Mouse CD41 Antibody (S-R690) Size:100T 5) Read the fluorescent signalProduct Specification Host Rat Antigen CD41 Synonyms Integrin alpha IIb; GPalpha IIb (GPIIb); Platelet membrane glycoprotein IIb; Itga2b Location Membrane Accession Q9QUM0 Clone Number S R690 Antibody Type Rat mAb Isotype IgG1,k Application FCM Reactivity Ms Positive Sample C57BL 6 mouse platelets Purification Protein G Concentration 0. 2 mg ml Conjugation Alexa Fluor 488 Physical Appearance Liquid Storage Buffer PBS, 1% BSA, 0. 3% Proclin 300
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