GFP His Tag protein, Aequorea victoria Size:50μg 4) Return to the standard

4) Return to the standard curve to calculate to calculate when diluted multiples (x 40)

CD152 Accession P16410 Amino Acid Sequence Ala37 - Phe162

(3) washing the cells twice with PBS and discarding the PBS

and then it was shaken at 4 ℃ for 20-25min or left to digest for 40-50min (observe the digestion at any time during the digestion)

GBP28 Accession Q15848 Amino Acid Sequence ETTTQGPGVLLPLPKGACTGWMAGIPGHPGHNGAPGRDGRDGTPGEKGEKGDPGLIGPKGDIGETGVPGAEGPRGFPGIQGRKGEPGEGAYVYRSAFSVGLETYVTIPNMPIRFTKIFYNQQNHYDGSTGKFHCNIPGLYYFAYHITVYMKDVKVSLFKKDKAMLFTYDQYQENNVDQASGSVLLHLEVGDQVWLQVYGEGERNGLYADNDNDSTFTGFLLYHDTNGGGSHHHHHH Expression System HEK293 Molecular Weight 36 kDa(Reducing) Purity ＞95%

GFP His Tag protein, Aequorea victoria Size:50μg 4) Return to the standardProduct Specification Species Aequorea victoria Antigen GFP Synonyms GFP Protein Accession AAB65663 Amino Acid Sequence Ser2 Lys238, with N terminal 8*His HHHHHHHHSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICTTGKLPVPWPTLVTTFSYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTISFKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIKANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEFVTAAGITHGMDELYK Expression System E. coli Molecular Weight 27. 8kDa